Figure 1.

(A) Study timeline. Explants of porcine retinae were prepared at day zero and cultivated for four and eight days. Degeneration processes were induced by adding CoCl₂ (300 µM) from day one to day three. Hypothermia treatment (30 °C) was applied simultaneously. Four groups were compared: control + 37 °C, CoCl₂ + 37 °C, hypothermia treated control + 30 °C and CoCl₂ + 30 °C. At days four and eight retina samples were prepared for immunohistological (IHC), western blot (WB) and qPCR analyses. (B) Hypothermia reduced the ROS-production in cultivated retina. ROS-level was measured 24 and 48 hours after CoCl₂-induction. For both points in time, the ROS-level was strongly elevated after CoCl₂-treatment. Hypothermia reduced the ROS-production significantly in CoCl₂-treated retinae. However, it was still higher than in control + 37 °C retinae. (C) pH-value was measured to assure that degenerative effects were induced by CoCl₂ and not by cultivation effects. pH-value was stable at any day of cultivation. B: n = 3/group. C: n = 10/group. **p < 0.01; ^###,***p < 0.001.