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. 2019 Mar 20;10(4):276. doi: 10.1038/s41419-019-1511-7

Fig. 1. Resveratrol inhibits decidualization.

Fig. 1

a RTQ-PCR analysis of PRL and IGFBP1 transcript levels in primary HESC cultures (n = 8) treated with cAMP and P4 in combination with or without resveratrol (100 µM) for 4 or 8 days. *P < 0.05; **P < 0.01. b Representative senescence-associated β-galactosidase (SAβG) staining of parallel cultures. Original magnification: ×100. Scale bar: 200 μm (left panel). SAβG staining intensity score calculated from 300 cells in three random fields. Data show fold-change (mean ± SEM) relative to vehicle control (right panel). c Representative Western blot analysis and quantification of p53 levels in whole-cell lysates from 4 independent cultures decidualized in the presence or absence of resveratrol for the indicated timepoints. β-actin serves as a loading control. d RTQ-PCR analysis of BTG2 transcript levels in primary HESCs treated as indicated (n = 7). Data show fold-change (mean ± SEM) relative to vehicle-treated undifferentiated cells (dotted line). Different letters above error bars denote significant difference at P < 0.05