Fig. 2. Resveratrol regulates SIRT1 expression in decidualizing HESCs.

a SIRT1 mRNA levels in undifferentiated and decidualized cells treated with cAMP and P4 in combination with or without resveratrol (100 µM) for 4 or 8 days. Data show fold-change (mean ± SEM) relative to undecidualized vehicle control (dotted line) of 8 independent experiments. b Representative Western blot and quantification of SIRT1 levels in whole cell lysates from 3 independent cultures treated in parallel. β-actin serves as a loading control. Different letters above error bars denote significance at P < 0.05. c Representative immunohistochemical images of SIRT1 expression in HESC cultures treated as indicated. Original magnification: ×200. Scale bar: 50 μm