Fig. 3. Impact of SIRT1 knockdown on resveratrol actions in decidualizing HESCs.

a RTQ-PCR analysis of SIRT1, PRL, and IGFBP1 transcript levels in HESC cultures first transfected with NT or SIRT1 siRNAs and then treated with cAMP and P4 in combination with or without resveratrol for 4 days. The experiment was carried out in 6 independent primary cultures. b Representative SAβG staining of parallel HESC cultures. Original magnification: ×100. Scale bar: 200 μm. SAβG staining intensity score was calculated from 300 cells in 3 random fields (right panel) and data show mean ± SEM score of 3 independent cultures. c Representative Western blot and quantification of SIRT1 and p53 proteins in whole-cell lysates obtained from parallel HESC cultures (n = 4). β-actin serves as a loading control. d RTQ-PCR analysis of BTG2 transcript levels in 6 independent primary cultures treated as indicated. Data show fold-change (mean ± SEM) relative to vehicle-treated undifferentiated cells (dotted line). Different letters above error bars denote significance at P < 0.05