Fig. 4. Resveratrol advances the downregulation of the CRABP2-RARα pathway in decidualizing HESCs.

a Representative Western blot and quantification of CRABP2, RARα, and PPARβ/δ proteins obtained from whole-cell lysates in undifferentiated or decidualized cells treated with cAMP and P4 in combination with or without resveratrol (100 µM) for the indicated timepoints (n = 3). β-actin serves as a loading control. b RTQ-PCR analysis of CRABP2, RARα, and PPARβ/δ transcript levels in HESC cultures (n = 6) first transfected with NT or SIRT1 siRNA and then treated with cAMP and P4 in combination with or without resveratrol for 4 days. c Representative Western blot and quantification of RA related genes in whole cell lysates (n = 3) from parallel primary cultures. β-actin serves as a loading control. Data show fold-change (mean ± SEM) relative to vehicle-treated undifferentiated cells (dotted line). Different letters above error bars denote significance at P < 0.05