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. 2019 Mar 20;9:4819. doi: 10.1038/s41598-019-41301-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EVTs are responsive to external stimulus TGFβ and undergo EMT. (A) 344SQ_EVTs are cultured in Matrigel for 7 days and treated with TGFβ (5 ng/ml) on day 3 (scale bar is 200 µM). In response to TGFβ, EVTs (B) hyper proliferate and (C) are more invasive. For each condition, 1000 structures were measured in size and scored for invasiveness. (D) Quantitative RT–PCR of indicated markers, shown as fold change upon TGFβ treatment. (E) Taqman RT–PCR assay of individual miR-200 members for EVTs in Matrigel and following TGFβ treatment. ***p < 0.0001. (F) Western blot analysis for EMT markers in Matrigel with or without TGFβ treatment.