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. 2019 Mar 18;9:4758. doi: 10.1038/s41598-019-41188-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometric analysis of phosphatidylserine (PS) exposure. Percentage of PS-exposing cells in the outer leaflet of the plasma membrane in each cell line. Untreated cells were stained with propidium iodide (PI) and Annexin V before flow cytometric analysis. The Annexin V stain is much used to detect apoptosis; however here we investigated untreated cells under the assumption that the PS observed in the outer leaflet was not a result of apoptosis but related to the composition of the cell membrane. Thus, Annexin V+/PI− show the percentage of cells exposing PS in the outer membrane. PI+ show the percentage of dead cells. Mean + SD, n = 3–4, each performed as individual experiments.