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. 2019 Mar 20;9:4747. doi: 10.1038/s41598-019-41066-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Calibration curve for varying glucose concentrations obtained with the tear-glucose device. The glucose solution was prepared by dissolving D-(+)-Glucose (Sigma-Aldrich, USA) at the concentrations of 0, 0.01, 0.05, 0.1, 0.2, 0.4 and 0.8 mM in phosphate-buffered saline (PBS) at pH 7.6, respectively. To collect 0.4 μl of the glucose solution, the ocular tip of the device was placed in contact with the glucose solution for 2 s. For measurement of the concentration of glucose, the tear-glucose device was connected to a potentiostat (DY2113, Digi-Ivy, USA) to apply 150 mV and the output current was obtained using the amperometric i-t curve mode.