KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-Aurora A (D3E4Q) | Cell Signaling Technology | Cat#14475S; RRID: AB_2665504 |
| Rabbit polyclonal anti-Aurora B/AIM1 | Cell Signaling Technology | Cat#3094S; RRID: AB_10695307 |
| Rabbit monoclonal anti-BTK (D3H5) | Cell Signaling Technology | Cat#8547S; RRID: AB_10950506 |
| Rabbit monoclonal anti-CDK4 (D9G3E) | Cell Signaling Technology | Cat#12790S; RRID: AB_2631166 |
| Mouse monoclonal anti-CDK6 (DCS83) | Cell Signaling Technology | Cat#3136T; RRID: AB_2229289 |
| Mouse monoclonal anti-CDK7 (C-5) | Santa Cruz Biotechnology | Cat#sc-365075; RRID: AB_10845930 |
| Rabbit polyclonal anti-CRBN | Novus Biologicals | Cat#NBP191810; RRID: AB_11037820 |
| Mouse monoclonal anti-FER (5D2) | Cell Signaling Technology | Cat#4268S; RRID: AB_2278286 |
| Rabbit polyclonal anti-FLT3 (C-20) | Santa Cruz Biotechnology | Cat#sc-479; RRID: AB_631052 |
| Rabbit monoclonal anti-GAPDH (14C10) | Cell Signaling Technology | Cat#2118S; RRID: AB_561053 |
| Mouse monoclonal anti-ITK (2F12) | Cell Signaling Technology | Cat#2380S; RRID: AB_10692901 |
| Mouse monoclonal anti-JAK1 (D1T6W) | Cell Signaling Technology | Cat#50996S |
| Rabbit monoclonal anti-JAK2 (D2E12) | Cell Signaling Technology | Cat#3230S; RRID: AB_2128522 |
| Rabbit monoclonal anti-LCK (D88) | Cell Signaling Technology | Cat#2984T; RRID: AB_2136313 |
| Rabbit monoclonal anti-LC3A/B (D3U4C) | Cell Signaling Technology | Cat#12741T; RRID: AB_2617131 |
| Rabbit polyclonal anti-Pyk2 (H364) | Cell Signaling Technology | Cat#3090S; RRID: AB_2174095 |
| Mouse monoclonal anti-α-tubulin (DM1A) | Cell Signaling Technology | Cat#3873S; RRID: AB_1904178 |
| Mouse monoclonal anti-vinculin (V284) | Sigma-Aldrich | Cat#SAB4200080; RRID: AB_10604160 |
| Rabbit monoclonal anti-WEE1 (D10D2) | Cell Signaling Technology | Cat#13084T; RRID: AB_2713924 |
| Rabbit polyclonal anti-ULK1 (A705) | Cell Signaling Technology | Cat#4776S; RRID: AB_2212518 |
| Rabbit monoclonal anti-phospho-STAT1 (Y701) (58D6) | Cell Signaling Technology | Cat#9167S; RRID: AB_561284 |
| Rabbit monoclonal anti-STAT1 (D4Y6Z) | Cell Signaling Technology | Cat#14995S |
| Goat IRDye 680RD anti-Rabbit IgG (H+L), 0.5 mg | LI-COR Biosciences | Cat#926-68071; RRID: AB_10956166 |
| Goat IRDye 800CW anti-Mouse IgG (H+L), 0.5 mg | LI-COR Biosciences | Cat#926-32210; RRID: AB_621842 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| cOmplete, Mini Protease Inhibitor Cocktail | Sigma-Aldrich | Cat#11836153001 |
| PhosSTOP Phosphatase Inhibitor Tablets | Sigma-Aldrich | Cat#04906837001 |
| recombinant human IFN-γ | R & D Systems | Cat#285-IF-100 |
| dTAG-7 | Erb et al., 2017 | N/A |
| MLN4924 | Active Biochem | Cat#A-1139 |
| pomalidomide | Selleckchem | Cat#S1567 |
| Critical Commercial Assays | ||
| CellTiter-Glo Luminescent Cell Viability Assay | Promega | Cat#G7571 |
| AlphaScreen Streptavidin Donor beads | PerkinElmer | Cat#6760002 |
| Nickel Chelate AlphaLISA Acceptor beads | PerkinElmer | Cat#AL108 |
| Deposited Data | ||
| Multiplexed Proteomics data in MOLM-14 cells | This paper |
https://www.ebi.ac.uk/pride/archive/ PRIDE: PXD007851 |
| Multiplexed Proteomics data in MOLT-4 cells | This paper |
https://www.ebi.ac.uk/pride/archive/ PRIDE: PXD007861 |
| Original, unprocessed data | This paper | http://dx.doi.org/10.17632/wbbbfhg5bj.1 |
| Experimental Models: Cell Lines | ||
| Human: MOLM-14 cells (male) | Laboratory of Scott Armstrong | RRID:CVCL_7916 |
| Human: MOLT-4 cells (male) | Laboratory of James Bradner | RRID:CVCL_0013 |
| Human: MV4-11 cells (male) | Laboratory of Scott Armstrong | RRID:CVCL_0064 |
| Human: TMD8 cells (male) | Belfer Center for Applied Cancer Science | RRID:CVCL_A442 |
| Human: 293FT cells (female) | ATCC | RRID:CVCL_6911 |
| Truchoplusia ni: High Five cells | ThermoFisher Scientific | Cat#B85502; RRID: CVCL_C190 |
| Experimental Models: Organisms/Strains | ||
| Oligonucleotides | ||
| sgCRBN-494 Forward 5′-CACCGTAAACAGACATGGCCGGCGA-3′ | Laboratory of James Bradner | N/A |
| sgCRBN-494 Reverse 5′-AAACTCGCCGGCCATGTCTGTTTAC-3′ | Laboratory of James Bradner | N/A |
| sgCRBN-496 Forward 5′-CACCGATATGCCTATCGAGAAGAAC-3′ | Laboratory of James Bradner | N/A |
| sgCRBN-496 Reverse 5′-AAACGTTCTTCTCGATAGGCATATC-3′ | Laboratory of James Bradner | N/A |
| Genotyping primer Forward (for locus targeted by 494): 5′-CGTGAAGCAGCTTTCCGGTG-3′ | Laboratory of James Bradner | N/A |
| Genotyping primer Reverse (for locus targeted by 494): 5′-GCAGGGGACGGCGTTCTGTC-3′ | Laboratory of James Bradner | N/A |
| Genotyping primer Forward (for locus targeted by 496): 5′-GATGCATAGCAAGGTTGGAAACCAC-3′ | Laboratory of James Bradner | N/A |
| Genotyping primer Reverse (for locus targeted by 496): 5′-CAACGCAGCGAGACTCCATC-3′ | Laboratory of James Bradner | N/A |
| Recombinant DNA | ||
| Plasmid: pSpCas9(BB)-2A-GFP (PX458) | Ran et al., 2013 | Addgene Plasmid #48138 |
| Plasmid: pFastBac vectors encoding CRBN and DDB1 | Fischer et al., 2014; Laboratory of Nicolas Thomä | N/A |
| Plasmid: Nluc/Fluc plasmid | Lu et al., 2015; Laboratory of Willian Kaelin | N/A |
| Software and Algorithms | ||
| R | R Core Team, 2016 | https://www.r-project.org/ |
| Limma | Ritchie et al., 2015 | https://bioconductor.org/packages/release/bioc/html/limma.html |
| GraphPad Prism 7.0a | GraphPad Software, Inc. | http://www.graphpad.com/ |
| Other | ||