Fig. 2.

Protein and mRNA abundance relationships. (A,) and (B,) Scatterplots of normalized mRNA and protein abundance for MAG transcripts and ejaculate-specific proteins (A,) and testis transcripts and sperm proteins (B,). The line and gray shading represent a linear model fit with its 95% confidence interval respectively. Coefficients of determination (R²,) are indicated. (C,) and (D,) Volcano plots of protein abundance differences for all proteins detected in both the ejaculate and sperm samples. Proteins that show >2-fold mRNA expression-bias in MAG or testes tissue are indicated in blue (MAG-biased) or red (testes-biased), and the size of each point corresponds to the number of unique peptides detected for each protein.