Table I. Gene Ontology analysis of sperm proteins, high-confidence SFPs, and sperm/SFP overlap.
List of significant terms is abbreviated to exclude redundancy and to focus on terms discussed in text. For exhaustive list, see Table S3. FDR; false discovery rate.
| Data set | Ontology | GO Term | Proteins (total in category) | FDR |
|---|---|---|---|---|
| Sperm Proteome | Biological Process | Organonitrogen compound metabolic process | 102 (978) | 5.3E-11 |
| Carboxylic acid metabolic process | 100 (553) | 1.9E-23 | ||
| Electron transport chain | 13 (55) | 6.5E-05 | ||
| Cellular Component | Mitochondrion | 150 (834) | 1.2E-45 | |
| Microtubule | 36 (210) | 1.3E-07 | ||
| Cilium | 32 (154) | 1.7E-08 | ||
| Proteasome complex | 31 (47) | 2.1E-25 | ||
| Dynein complex | 19 (40) | 1.7E-11 | ||
| Molecular Function | Nucleoside phosphate binding | 187 (1629) | 5.7E-20 | |
| Hydrolase activity | 163 (2153) | 4.7E-04 | ||
| Oxidoreductase activity | 103 (839) | 2.0E-13 | ||
| Threonine-type endopeptidase activity | 14 (15) | 8.3E-16 | ||
| High-confidence SFP proteome | Biological Process | Organonitrogen compound metabolic process | 30 (978) | 0.009 |
| Carboxylic acid metabolic process | 21 (553) | 0.033 | ||
| Hexose metabolic process | 10 (79) | 7.5E-04 | ||
| Cellular Component | Extracellular region part | 33 (1116) | 0.029 | |
| Molecular Function | Hydrolase activity | 62 (2153) | 0.000 | |
| Nucleoside phosphate binding | 43 (1629) | 0.040 | ||
| Peptidase activity | 27 (739) | 0.006 | ||
| Sperm/SFP overlap | Biological Process | Carboxylic acid metabolic process | 29 (553) | 7.2E-11 |
| Vesicle-mediated transport | 15 (536) | 0.017 | ||
| Protein folding | 10 (113) | 2.6E-05 | ||
| ATP hydrolysis coupled proton transport | 8 (25) | 4.3E-08 | ||
| Oocyte microtubule cytoskeleton polarization | 3 (10) | 0.014 | ||
| Molecular Function | Nucleoside phosphate binding | 39 (1629) | 3.4E-06 |