Table II. Potential antibacterial candidates identified in the heparin-binding fraction of the chicken amniotic fluid at ED11 and ED16. Proteins contained in the SDS-PAGE bands corresponding to bacterial inhibition zones (Listeria monocytogenes and Salmonella Enteritidis, Fig. 7) were analyzed using mass spectrometry. Bands where spectral counts were the highest are indicated in bold.
| Identified Proteins (29) | Accession Number | Gene symbol | Molecular Mass (kDa) | Corresponding SDS-PAGE bands (Fig. 7) |
|---|---|---|---|---|
| Mutant cysteine-rich FGF receptor [G. gallus,] | AAB39211.1 | GLG1 | 122 | G |
| Avidin [G. gallus,] | CAC34569.1 | AVD | 17 | B, C, D, E, F, G, H |
| OvoglobulinG2 type BB [G. gallus,] | BAM13273.1 | BPIFB2 | 47 | B, C, D, E, F |
| Ovomacroglobulin, ovostatin [G. gallus,] | CAA55385.1 | OVST | 164 | C |
| Sodium/potassium-transporting ATPase subunit alpha-1, precursor [G. gallus,] | NP_990852.1 | ATP1A1 | 112 | A |
| Peptidyl-prolyl cis-trans isomerase B precursor [G. gallus,] | NP_990792.1 | PPIB | 19 | E |
| Mucin-6, partial [G. gallus,] | XP_015142236.1 | MUC6 | 98 | B, C |
| Metalloproteinase inhibitor 2 precursor [G. gallus,] | NP_989629.1 | TIMP2 | 22 | E |
| Metalloproteinase inhibitor 3 precursor [G. gallus,] | NP_990818.1 | TIMP3 | 20 | E |
| Gallinacin-11 precursor [G. gallus,] | NP_001001779.1 | AvBD11 | 12 | C |
| Ovoinhibitor precursor [G. gallus,] | NP_001025783.2 | SPINK5 | 52 | B, C |
| Ovalbumin-related protein Y [G. gallus,] | NP_001026172.1 | OVALY | 44 | B, C, D |
| Elongation factor 1-alpha 1 [G. gallus,] | NP_001308445.1 | EEF1A1 | 50 | G |
| Vitelline membrane outer layer protein 1 precursor [G. gallus,] | NP_001161233.1 | VMO1 | 20 | C, E, F, G |
| Astacin-like metalloendopeptidase precursor [G. gallus,] | NP_001292019.1 | ASTL | 46 | D |
| Lysozyme C [G. gallus,] | P00698.1 | LYZ | 16 | A, B, C, D, E, F, G, H |
| Ovalbumin [G. gallus,] | P01012.2 | OVAL | 43 | A, B, C, D, E, F, G, H |
| Ovotransferrin [G. gallus,] | P02789.2 | TF | 78 | B, C |
| Ovocleidin-17 [G. gallus,] | Q9PRS8.2 | OC-17 | 15 | G |
| Ovomucin [G. gallus,] | XP_003641415.1 | MUC5B | 234 | B, C |
| Tumor necrosis factor receptor superfamily member 6B [G. gallus,] | XP_004947191.1 | TNFRSF6B | 38 | D |
| Beta-actin [G. gallus,] | CAA25004.1 | ACTB | 46 | A, G |
| Beta-microseminoprotein-like [N. notabilis], | XP_010010315.1, | LOC104403769, | 15, | C, E, F, G, |
| Ovalbumin-related protein X [G. gallus,] | XP_015137660.1 | OVALX | 45 | B, C, D, E, F, G |
| Clusterin [G. gallus,] | XP_015140573.1 | CLU | 62 | C |
| Midkine [G. gallus,] | XP_015142525.1 | MDK | 16 | G |
| Alpha-2-macroglobulin-like protein 1 [G. gallus,] | XP_015148230.1 | A2ML1 | 160 | B, C |
| ATP synthase subunit alpha, mitochondrial [G. gallus,] | NP_989617.1 | ATP5A1Z | 56 | A |
| Deleted in malignant brain tumors 1 protein-like [G. gallus,] | XP_015156102.1 | DMBT1L3 | 100 | B, C |
In italics,: LOC104103769 from N. Notabilis, has a G. Gallus, homologue (LOC101750704), which has been erroneously removed from Pubmed database following the release of Gallus_gallus-5.0 assembly in 2015. This chicken homolog has been unambiguously identified in egg white (25) and shares 88.7% sequence identity with LOC104403769 from N. notabilis,.