Fig. 3.

Conservation of the crocodile sperm proteome and annotation of proteins not curated within the compiled human sperm proteome. A,, B,, Venn diagrams illustrating the conservation of crocodile sperm proteins with those reported for (A,) human (27) and (B,) rooster spermatozoa (28). C–E,, Gene Ontology (GO) analysis was performed to assess the functional classification of the 130 crocodile sperm proteins that are not currently annotated within the compiled human sperm proteome. For this purpose, proteins were curated on the basis of (C,) GO molecular function, (D,) GO biological process, and (E,) GO cellular compartment using the universal protein knowledgebase (UniProtKB) functional annotation tools (26).