Fig. 7.

Plots depicting fold changes associated with differentially phosphorylated peptides. Plots were constructed to demonstrate the fold change (x, axis; depicted as log₂ fold change) and overall SequestHT Score (y, axis) of phosphorylated peptides in (A,) membrane-enriched and (B,) soluble lysates extracted from noncapacitated and capacitated crocodile spermatozoa. For the purpose of this analysis, a threshold of at least a ± 1.2 fold change in reporter ion intensity was implemented to identify differentially phosphorylated peptides. The identity of the parent protein from which a portion of the peptides originate has been annotated.