Figure 1.

Biological characterisation of 1h. The global reciprocal plot of data from MbtI steady-state kinetics analysis towards chorismic acid, in the presence of different concentrations of 1h (A), highlights the competitive behaviour of the inhibitor. IC₅₀ plots of 1h in the presence of BSA (green squares), Triton X-100 (red triangles) and DTT (yellow diamonds) (B) confirm that the compound is not a PAIN. The Universal CAS assay performed on culture media of M. bovis BCG cells grown in the presence of 1h (C), together with the determination of the mycobactins in the above-mentioned cells (D), confirm that the antimycobacterial activity is related to iron uptake inhibition. All data are mean ± SD of three replicates.