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Schematic diagram of the sensing principle based on the redox cycling of [Fe(CN)6]3−/[Fe(CN)6]4− between enzyme and electrode surfaces at nano-sized gold nanoparticle (Au-NPs)/carbon interdigitated electrodes (IDEs), selectively functionalized with cholesterol oxidase (ChOx); Working electrodes (comb 1: enzyme-functionalized Au-NPs/carbon comb, comb 2: non-functionalized Au-NPs/carbon comb. Image reproduced from [44] with permission.
