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. 2019 Mar 14;12:62. doi: 10.3389/fnmol.2019.00062

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Copyright © 2019 Wever, Largo-Barrientos, Hoekstra and Smidt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The number of TH+ cells is decreased in Pitx3Cre/+; Lmx1b L/L 3-month-old midbrains. (A) Protein expression of TH (green) was evaluated via immunohistochemistry in the adult midbrain of 3-month-old Lmx1b cKO animals. A loss of TH signal was observed in both the ventral tegmental area (VTA) and the substantia nigra pars compacta (SNc; white arrowheads). The white dotted line represents the border between what is considered SNc and VTA. (B) Quantification of TH+ cells in the adult midbrain of Pitx3Cre/+; Lmx1b L/L (n = 3, gray bars) and Pitx3Cre/+; Lmx1b +/+ controls (n = 3, black bars) shows that the total amount of TH+ neurons is significantly lower (~ 15% loss, **P < 0.05, one-tailed) and that neurons are lost both in the SNc (~ 13% loss, *P < 0.05) and the VTA (~ 20% loss, **P < 0.01, one-tailed). Pitx3Cre/+ Lmx1b+/+ animals were set at 1. Scale bar = 100 μM.