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. 2019 Mar 14;12:62. doi: 10.3389/fnmol.2019.00062

Figure 9.

Figure 9

The TH+ cells that are lost in the SNc of 3-month-old mice conditionally depleted of Lmx1b are also AHD2+, while the TH+AHD2- population is affected in the VTA. Immunohistochemistry of TH (green) and AHD2 (red) in the adult midbrain of 3-month-old animals. (A) Assessment of the TH+AHD2+ (yellow) and the TH+AHD2- (green) population in both the SNc and VTA of Pitx3Cre/+ Lmx1b L/L animals shows that in the SNc (upper panel) the TH+ AHD2+ neurons are affected, while TH+ neurons affected in the VTA (lower panel) are AHD2- (white arrowheads). The white dotted line represents the border between what is considered SNc and VTA. (B) Quantification of the amount of cells in the SNc shows that the number of TH+AHD2+ neurons are reduced (~18%, n = 3, *p < 0.05, one-tailed), while the number TH+AHD2- cells are similar to the Pitx3Cre/+ Lmx1b +/+ (n = 3, black bar). (C) This in contrast to the VTA, where TH+AHD2- cells are lost (~ 21% loss, n = 3, **P < 0.01, one-tailed) in the Lmx1b cKO (gray bar) and TH+AHD2+ cells are not significantly reduced. The number of cells of in the midbrain of Pitx3Cre/+; Lmx1b +/+ animals were set at 1. Scale bar = 100 μM.