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. 2019 Mar 21;12:61. doi: 10.1186/s13068-019-1404-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Cell growth of the acetate sensitive knockouts. K. phaffii phenotypes of wild-type GS115 (WT), ΔPAS_chr3_1091 (ΔHRK1), ΔPAS_chr4_0402, and ΔPAS_chr4_0812 are shown. Cells were spotted on YPD agar plates (pH 4.5) with acetate of 0, 30 and 40 mM at three concentrations (OD₆₀₀ = 0.1, 0.01 and 0.001). These strains were selected from among the 92 kinase gene knockouts (Additional file 1: Fig. S2)