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Cell lines expressing tagged-proteasome subunits
| Tag | Tagged-Subunit | Strain | Source | Reference |
|---|---|---|---|---|
| 3XFLAG | Dss1 | HEK293F | Human | (13) |
| 3XFLAG | β4 | Myoblast | Human | |
| HTBH* | Rpn11 | HEK293 | Human | (12) |
| 3xFLAG | Rpn11 | YYS40 | S. cerevisiae | (10) |
| Protein A | Rpn11 | SDL66 | S. cerevisiae | (11) |
| Protein A | β4 | Y-SDL135 | S. cerevisiae | (9) |
The HTBH tag consists of two hexahistidine tags, a TEV cleavage site, and a bacterially derived peptide that induces biotinylation in vivo (12).
