| Templates for in vitro transcription of sgRNAs | |
|---|---|
| thraa | taatacgactcactataGGAGCGGTAATGATAGCCAGgttttagagctagaa |
| thrab | taatacgactcactataGGGAAAGAACAGCCAGTGTTgttttagagctagaa |
| l-thrb+ | taatacgactcactataGGGTGAGTTATGCACCATGGgttttagagctagaa |
| thrb* | taatacgactcactataGGGAGAACCGTGAACGCCGAgttttagagctagaa |
| Generic for template assembly | AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTaacttgctatttctagctctaaaac |
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