Fig. 1. The MDS concept. (A) Monomers form proteins that have a single epitope that is captured by an antibody (capturing antibody: 6E10) attached to the surface of the plate. After adding a detection antibody (FF51-HRP), monomer proteins cannot be detected because the single epitope is already occupied. (B) Multimers with numerous epitopes can be detected by detection antibodies. The capturing and detection antibodies are different, but their epitopes overlap.
MDS: Multimer Detection System.