Figure S5. GluA1 expression and AMPA responses are decreased in AAV-ShPpara–transduced cortical cells.

(A, B) Cortical cultures transduced with AAV containing an shRNA sequence targeting Ppara (AAV-ShPpara). An AAV-scramble shRNA (AAV-ShSc) was used as a control. The cells were immunolabelled for PPARα (red) and MAP2 (blue, neuronal marker). Scale bar, 200 μm. In (A) PPARα quantification in AAV-ShSc or AAV-ShPpara transduced cortical cells. Data are normalized to AAV-ShSc (n = 6 of each analyzed in three independent experiments, *P < 0.05, Mann–Whitney test). (B) Recordings of calcium transients in one representative GFP positive cell (green) transduced with AAV-ShSc or AAV-ShPpara (insets). Scale bar: 5 μm. (C) AMPA-induced calcium fluorescence in AAV-ShSc and AAV-ShPpara cortical cells. Insets: AMPA responses amplitude expressed as ΔF/F0 (AAV-ShSc; n = 104, AAV-ShPpara; n = 108 cells analyzed in three independent experiments, respectively; ***P < 0.001, Mann–Whitney test). (D) Left panel: RT-qPCR analyses of Gria1 and Abca1 mRNA levels in AAV-ShSc or AAV-ShPpara–transduced cells (n = 9 of each analyzed in four independent experiments). Results are expressed as percentage of respective AAV-ShSc (GluA1: ***P < 0.001, Mann–Whitney test, ABCA1: P > 0.05, t test). Middle panel: representative Western blot of corresponding proteins. Right panel: GluA1 and ABCA1/α tubulin ratios. Results are expressed as percentage of respective AAV-ShSc (n = 6 of each analyzed in three independent experiments, ***P < 0.001, t test). Data information: data are presented as mean ± SEM.

Source data are available for this figure.