Figure S7. PPARα and GluA1 expression in male and female Tg mice.

(A) Comparative RT-qPCR analyses of Ppara and Gria1 (encoding PPARα and GluA1, respectively) mRNA levels in the hippocampus from male (♂) and female (♀) 5xFAD transgenic (Tg) mice at 9–10 mo. Results were normalized to Gapdh mRNA and are expressed as percentage of corresponding Tg ♀ (♂ and ♀: PPARα n = 5; GluA1 n = 6 of each, *P < 0.05, **P < 0.01; t test). PPARα expression was visualized by semi-quantitative RT-PCR (right panel). (B) Representative Western blot of hippocampal lysates from female (♀) and male (♂) Tg mice. Expression of GluA1 was monitored with the anti-GluA1 antibody and blots were further probed using anti-α tubulin antibody. Right panel: quantification of GluA1/α tubulin ratios. Results are expressed as percentage of Tg ♀ (n = 4 for each condition, **P < 0.01, t test). (C) CA1 LTP in hippocampal slices from male (♂) transgenic (Tg) 5xFAD mice (9–10 mo) perfused with 4 μM bexarotene (bex, n = 4) or with vehicle (veh, n = 2), ***P < 0.001, t test. Data information: data are presented as mean ± SEM.

Source data are available for this figure.