Figure 1.

Characterization of cDC subsets in hepatic and inguinal lymph nodes, spleen, and liver. (A) Vital (7-AAD⁻)CD45⁺Lineage⁻CD1c⁺ and CD45⁺Lineage⁻CD141⁺cells were gated in MNC isolated from hepatic and inguinal lymph nodes, spleen and liver perfusate, and analyzed for CD11c expression. (B) Vital (7-AAD⁻)CD45⁺Lineage⁻CD141^bright cells were analyzed for Clec9A expression. Cells isolated from lymphoid tissues showed low Clec9A expression, while their counterparts in liver perfusate were Clec9A⁺. When liver perfusate cells were incubated with collagenase, Clec9A expression was lost. When leukocytes were isolated from inguinal LN without collagenase digestion, Clec9A was expressed on cDC2. iLN, inguinal LN.