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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: Cancer Res. 2019 Jan 9;79(6):1124–1137. doi: 10.1158/0008-5472.CAN-18-1753

Figure 5. Induction of exogenous AR down-regulates expression of stem cell-associated markers and promotes luminal epithelial cell fate.

Figure 5.

(A) HPET cells and HuSLCs were transfected with 30 μg and 40 μg of pSVARo respectively and treated with/without DHT with/without OHF as indicated. Expression of the stem cell factors Oct4, Nanog, Sox2, Nestin and CD44 were determined by RT-qPCR.

(B) Cells were transfected and treated as in (A) and expression of prostate luminal epithelial cells factors PSA, PAP, Nkx3.1, TMPRSS2 and FGF5 were determined by RT-qPCR.

(C) Cells were transfected and treated as in (A) and expression of p63 and ChrA were determined by RT-qPCR. Primer sets to characterize expression of all of these factors are listed in Supplementary Table S3. Data are expressed as mean ± SEM; n=4. *, p<0.01; **, p<0.001; ***, p<0.0001.