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. 2019 Mar 10;11(5):1333–1341. doi: 10.18632/aging.101830

Figure 2.

Figure 2

PTTG3P is upstream of PTTG1 in CC. (A-B) We overexpressed PTTG1 or depleted PTTG1 (by expressing short hairpin interfering RNA for PTTG1-shPTTG1) in two CC lines, Ca-Ski and HTB31. Plasmids carrying a scrambled sequence (SCR) were used as controls. (A) RT-qPCR for PTTG1. (B) RT-qPCR for PTTG3P. (C-D) We overexpressed PTTG3P or depleted PTTG3P (by expressing short hairpin interfering RNA for PTTG3P-shPTTG3P) in two CC lines, Ca-Ski and HTB31. (C) RT-qPCR for PTTG3P. (D) RT-qPCR for PTTG1. *p<0.05 (versus SCR). NS: non-significant. N=5.