Figure 1. Flowchart depicting the pipeline implemented in this study for analysis and annotation of the An. darlingi mobilome. The assembled genome (ADMH02000000) was screened for TEs by similarity-based approach, using the Repbase database by Blastn and tblastx. In parallel, the genome was screened de novo by using Repeatscout. Redundancies were removed and TEs were classified using Censor with the Repbase database. Also, a manual annotation was performed, using UGENE, with emphasis on TIRs, LTRs, TSDs and ORFs. The content of TEs in the genome was calculated using RepeatMasker.