Figure 1. Primer location and specificity for qPCR analysis. (A) Primer pair sequence for STX1A (NC_000007.14) (FP 5’ TTAAAGAGCATCGAGCAGTCC 3’ and RP 5’ CGACATGACCTCCACAAACTC 3’), Amplicon size is 120 bp, TM=62 °C, location at GRCh38.p12; (Chr7: 73704218-73704237) (Chr7: 73704423-73704410). (B) VAMP2 (NC_000017.11) primer pair sequence; forward primer (5’ GTGGACATCATGAGGGTGAA 3’), reverse primer (5’GTATTTGCGCTTGAGCTTGG 3’). Amplicon size is 138 bp, TM=55 °C, location GRCh38.p12; (Chr17: 8161626-8161645), (Chr17: 8161763-8161744).
