Figure 2. Genes involved in the processing of microRNAs-like are induced in the parasitic phase of P. brasiliensis. Quantitative RT-PCR was performed with transcripts of Pb18 yeast cells cultivated in liquid Sabouraud medium for 18 hours. Expression values of genes involved in the processing of microRNAs (dcr-1, dcr-2, ago-1, ago-2) were calculated using actin as the endogenous control. Data are expressed as mean ? standard deviation from triplicates.