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. 2019 Mar 21;14(3):e0214059. doi: 10.1371/journal.pone.0214059

Fig 2. Tax-1 Interaction with SNX27 was Dependent on Tax-1 PBM and SNX27 PDZ Domains.

Fig 2

A) S-tag pull-down demonstrating importance of Tax-1 PBM for interaction with SNX27. HEK293T cells were transfected with 10 μg of indicated S-tag construct (empty, Tax-1, or Tax-1 ΔPBM). 24 hours post transfection S-tag pull-downs were performed. Samples were subjected to SDS-PAGE followed by immunoblotting and probed for SNX27 and S-tag, as shown. B and C) Co-IP of SNX27 to demonstrate the importance of the SNX27 PDZ domain in the interaction of Tax-1 and SNX27. HEK293T cells were transfected with 5 μg either Myc-SNX27 WT (B) or Myc-SNX27 ΔPDZ (C) along with 5 μg of indicated S-tag constructs (empty, Tax-1, or Tax-1 ΔPBM). 24 hours post transfection, cells were collected, and lysates were subjected to co-IP using either Myc or normal IgG antibodies. Samples were subjected to SDS-PAGE followed by immunoblotting and probed for Myc and S-tag, as shown.