Table 4. Gene-specific primers used for analysis of expression of HgNR, HgNiR, HgGS, HgGDH, and HgAS.

Primer	Sequence Fragment
HgAS-F	ATTTGGAGAGGACAGGGTACCATGTGTGGCATACTTGCTGT
HgAS-R	GGTACTAGTGTCGACTCTAGACGAGCTAGTGATTGCAAGC
HgGDH-F	ATTTGGAGAGGACAGGGTACCATGAATGCATTAGTTGCTAC
HgGDH-R	GGTACTAGTGTCGACTCTAGAAGCTTCCCAACCTCTGAGA
HgGS-F	ATTTGGAGAGGACAGGGTACCATGGCGCAGATTTTGGCAC
HgGS-R	GGTACTAGTGTCGACTCTAGAGACATTCAATGCCAACTTC
HgNiR-F	ATTTGGAGAGGACAGGGTACCATGTCATCATCATCATCGTC
HgNiR-R	GGTACTAGTGTCGACTCTAGAGCAGTCTTCTGCCTCTTC
HgNR-F	ATTTGGAGAGGACAGGGTACCATGTCATCATCATCATCGTC
HgNR-R	GGTACTAGTGTCGACTCTAGAGCAGTCTTCTGCCTCTTC

The underlined portion of each primer indicates the restriction enzyme sites for Kpn I and Xba I.