Figure 4.

Invasive Duct Carcinoma, Grade III with CEP 17 Polysomy and Amplified TOP2A Gene Copy Number. (A), Histologic section of the tumor, Hx&E stained (X200); (B), Immunostained slide for KI-67 LI, showing high proliferation rate 90%, (x200). (C), Representative FISH image, showing CEP17 polysomy and amplified TOP2A gene copy number (as demonstrated by 4-5 aqua signals for the chromosome “red arrows” & red clusters for the gene denoting high amplification pattern “yellow arrows”). Gene to chromosome ratio is >2.