Figure 5.

Camel Milk Induces Autophagy. (a, b): Light microscopy images showing comparative morphological changes in HCT 116 and MCF-7 cells untreated or treated with camel milk for 24h. The black arrows indicate the presence of vesicles. Scale bar: 15 µm. (c, d): Confocal microscopy images showing the localization of GFP-LC3 puncta (white arrows) in HCT 116 and MCF-7 cells treated with camel milk in compared to the diffused fluorescence in the untreated cells. Cells were transiently transfected with pEGFP-LC3 plasmid for 24h and then the GFP-LC3 positive cells were treated with or without 100 µL/mL camel milk for additional 24h. Fluorescence images were captured at 60x magnification, scale bar: 20 µm.