Figure 5. Sleep promoting vFB neurons are sufficient and necessary for LTM consolidation.

(A) SIs of males of indicated genotypes tested 24 hr after 1 hr training with a mated female and activation at the specific time interval with CsChrimson (orange bars). SI of control males with relevant neurons not activated (green bar). P value is for Ho SI = 0; ***p<0.001, **p<0.01, *p<0.05, ns p>0.05. Permutation test. (B) SIs of males of indicated genotypes tested 24 hr after training for 6 hr with a mated female and silencing with shi^ts at the specific time interval (dark blue bars). SI of wild type control males with DAN-aSP13 active (green bar). P value is for Ho SI = 0; ***p<0.001, **p<0.01, *p<0.05, ns p>0.05. Permutation test.

Figure 5—figure supplement 5. Courtship Indices (CIs) of males that had undergone treatment according to Figure 5A and B.

(A) Courtship indices (CIs) of males trained in single pair assays with a mated female and with FB neurons activated as indicated in Figure 5A and Table S9. (B) Courtship indices (CIs) of males trained in single pair assays with a mated female and with FB neurons silenced as indicated in Figure 5B and Table S10.