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. Author manuscript; available in PMC: 2019 Mar 21.
Published in final edited form as: Science. 2014 Sep 19;345(6203):1512–1515. doi: 10.1126/science.1251550

Fig. 3. IRBIT stabilizes and inhibits RNR-dATP complex.

Fig. 3.

(A) IRBIT does not create additional dATP binding sites on RNR. RNR was mixed with increasing doses of [32P]dATP in the absence (circles) or presence (squares) of hIRBIT and in the presence of dTTP (1 mM) at +4°C. (B) IRBIT inhibits dATP release from RNR. Values are means T SEM. (C) Rate constants of R1-dATP interaction. Bmax (maximum number of binding sites) was derived from (A), koff was obtained from (B), and kon was calculated from fig. S2D. Kd, dissociation constant. (D) IRBIT enhances dATP inhibition of RNR. RNR was premixed with or without dATP and with or without 6His-IRBIT and tested for uridine diphosphate (UDP) reductase activity.