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. Author manuscript; available in PMC: 2019 Mar 21.
Published in final edited form as: Science. 2014 Sep 19;345(6203):1512–1515. doi: 10.1126/science.1251550

Fig. 4. IRBIT controls the endogenous dNTP pool and is required for normal cell cycle progression.

Fig. 4.

(A) dNTP levels in HeLa and in HeLaDIRBIT cells in mitotic (M) or G1 phases; a/s for asynchronous population. (B) Stability of R1 and IRBIT do not depend on each other. Total lysates of HeLa, HeLaDIRBIT, and HeLaDR1 cells were analyzed by Western blots using antibodies against IRBIT, R1, and R2B. (C) IRBIT-RNR interaction during the cell cycle. IRBIT was immunoprecipitated from corresponding lysates and analyzed for the presence of R1. (D) Cell cycle progression of HeLa cells with or without IRBIT. M-to-M transition indicates timing between anaphase and prometaphase of the next cell cycle of an individual daughter cell (n = 20). Values are means T SEM. Statistical differences were evaluated by Student’s t test (**P < 0.01).