Figure 4 A-C.

Metallosis patients demonstrate downregulation of extracellular matrix proteins and maintenance enzymes. Gene set enrichment analysis was performed on the 667 downregulated genes identified in Fig. 1D. Overlap of this gene set with curated gene sets was calculated and the 10 most enriched gene lists are displayed with false discover rate (FDR) q-value shown (A). STRING protein-protein interaction analysis was conducted with a subset of genes (n=69) associated with the “Extracellular Matrix” gene list (B). All collagen genes within this gene list were extracted, and hierarchical clustering was performed using the RPKM values for these genes after a log2 adjustment (C).