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. Author manuscript; available in PMC: 2019 Mar 21.
Published in final edited form as: Cell Rep. 2017 May 30;19(9):1902–1916. doi: 10.1016/j.celrep.2017.05.019

Figure 4. Vascular Tube-Stabilizing Potential of MB-Derived Mural Cells.

Figure 4.

(A) HUVECs were cultured alone or cocultured with H9-EGFP hESC-derived MSCs, imPCs, or PKH67-labeled brain PCs (brPC) in pre-solidified Matrigel in EGM2 media. The cells were then photographed at the indicated time points using fluorescent microscope. Scale bar, 100 μm.

(B) HUVECs were cocultured with H9-EGFP hESC-derived PC1 and PC2 photographed using fluorescent microscope at the indicated time points. Scale bar,100 μm.

(C) Close-up view depicting tubular structures formed by HUVECs that are closely associated with PC1. Arrows point to PCs co-aligned with endothelial tubes. Scale bar, 10 μm.

(D) 3D volumetric image of tubules formed by HUVECs in presence of PC1 (see also Movie S2).

(E) HUVECs were cocultured with H9-EGFP hESC-derived imSMCs and mSMCs photographed using fluorescent microscope at the indicated time points. Scale bar, 100 μm.

(F and G) Quantification of cumulative tube length (F) and retention of cumulative tube length (G) at indicated time intervals. Results are mean ± SE of three independent studies (*p < 0.01).