Table 5.
Estimation of the MAP spiking levels L0, L1, L2, and L3 in milk test portions prepared for the LOD study by MPN technique expressed in log MPN/50 ml.
| Dairy product (Strain spiked) | Method to be tested | L0 | L1 | L2 | L3 |
|---|---|---|---|---|---|
| Raw milk | Culture | 0 | 3.88 | 4.88 | 5.18 |
| (ATCC 19851)1 | IS900 and f57 qPCR | 0 | 3.18 | 3.88 | 5.18 |
| PMS-phage | 0 | 3.18 | 3.88 | 5.18 | |
| Heat-treated | Culture | 0 | 2.32 | 3.32 | 5.02 |
| milk | IS900 and f57 qPCR | 0 | 3.02 | 3.32 | 5.02 |
| (DSM 44135) | PMS-phage | 0 | 2.32 | 3.32 | 5.02 |
| Milk powder | Culture | 0 | 2.85 | 3.54 | 4.54 |
| (DSM 44133) | IS900 and f57 qPCR | 0 | 2.85 | 3.54 | 4.54 |
| PMS-phage | 0 | 2.85 | 3.54 | 4.54 |
Values are expressed considering the lowest dilution factor applied. 1Estimated by MPN qPCR.