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. 2019 Mar 15;10:457. doi: 10.3389/fimmu.2019.00457

Figure 3.

Figure 3

Arid3a KO mice exhibit adult-type B cell development without B1a cell generation. (A) Arid3a qRT-PCR of B-lineage in CD2-Cre+ Arid3a WT mice in d0-1 liver and 2 mo BM. n = 2 each; mean. (B) Arid3a PCR of 2 mo BM B-lineage and spleen FO B cells in CD2-Cre+ Arid3a WT and KO littermates. (C) MHC class II staining of pre-B and immature B cells in d0 Arid3a KO and WT littermates. Represent of 3 mice each. All showed similar increases in Arid3a KO compared to WT. (D) Neonatal spleen (day 5) and PerC (day 10) analysis of Arid3a KO and WT littermates, and CD5 level comparison in B cells (CD19+). Three mice each showed similar results. (E,F) Adult (2 mo) Arid3a KO and WT spleen and PerC analysis. B1a cells in PerC are marked. Percentage of total B cells in spl and PerC in lymphoid area (n = 4 each; mean ± s.e.) and B1a cells in total B cells in PerC. (G) Arid3a deficient mouse generates B1b, MZ B, and FO B cells, and loss of B1a cell generation. (H) PBL crossed with TCL1 Tg-CD2-Cre+ mice. CLL/lymphoma incidence difference in TCL1 Tg expressing Arid3a KO vs WT littermates.