TABLE 1.
Strain or plasmid | Relevant characteristic | Reference or source |
---|---|---|
S. aureus strains | ||
RN4220 | Restriction-deficient transformation recipient | 36 |
TCH1516 | Community-associated USA300 MRSA isolate | 37 |
UAMS-1 | Osteomyelitis clinical isolate | 38 |
SH1000 | rsbU repaired laboratory strain | 39 |
Newman | Laboratory strain | 40 |
AH1263 | USA300 LAC isolate cured of plasmid LAC-p03 | 41 |
JE2 | USA300 LAC isolate cured of plasmids LAC-p01 and LAC-p03 | 42 |
RKC0602 | UAMS-1 pMK4 | This work |
RKC0603 | UAMS-1 pMK4_Teg41 | This work |
RKC0600 | SH1000 pMK4 | This work |
RKC0601 | SH1000 pMK4_Teg41 | This work |
RKC0535 | AH1263 pMK4_Teg41 | This work |
RKC0604 | Newman pMK4 | This work |
RKC0605 | Newman pMK4_Teg41 | This work |
RKC0072 | TCH1516 pMK4 | This work |
RKC0474 | TCH1516 pMK4_Teg41 | This work |
RKC0494 | JE2 pMK4 | This work |
RKC0470 | JE2 pMK4_Teg41 | This work |
RKC0599 | AH1263 pMK4 | This work |
RKC0538 | AH1263 pMK4_Teg41 | This work |
RKC0630 | AH1263 pCN51 | This work |
RKC0614 | AH1263 pCN51_Teg41 | This work |
RKC0628 | AH1263 pCN51_Teg41_3′ | This work |
RKC0552 | AH1263 pMK4_Teg41_5′ | This work |
NE1354 | USA300 JE2 hla::Bursa, hla NARSA transposon mutant | 42 |
RKC0183 | TCH1516 hla::Bursa, hla mutant | This work |
RKC0472 | TCH1516 hla::Bursa pMK4_Teg41 | This work |
RKC0521 | AH1263 hla::Bursa, hla mutant | This work |
BB2373 | JE2 Δpsmα | B. Boles |
RKC0504 | JE2 Δpsmα pMK4 | This work |
RKC0442 | JE2 Δpsmα pMK4_Teg41 | This work |
JLB162 | AH1263 Teg41 3′ deletion (Teg41Δ3′) | This work |
RKC0535 | JLB162 pMK4_Teg41 | This work |
RKC0553 | JLB162 pMK4_Teg41_5′ | This work |
RKC0631 | JLB162 pCN51 | This work |
RKC0615 | JLB162 pCN51_Teg41 | This work |
RKC0629 | JLB162 pCN51_Teg41_3′ | This work |
S. epidermidis strains | ||
1457 | Wild-type S. epidermidis | 43 |
RKC0518 | 1457 pMK4 | This work |
RKC0519 | 1457 pMK4_Teg41 | This work |
E. coli strain | ||
DH5α | Cloning strain | Invitrogen |
Plasmids | ||
pMK4 | Gram-positive shuttle vector (Cmr) | 44 |
pRKC0486 | pMK4_Teg41 (vector overexpressing Teg41 from its native promoter) | This work |
pRKC0554 | pMK4_Teg41_5′ (vector overexpressing the 5′end of Teg41 from its native promoter, i.e., Teg41 missing the 3′ end) |
This work |
pCN51 | Cadmium-inducible expression vector | 45 |
pRKC0473 | pCN51_Teg41 (vector overexpressing Teg41 from an inducible promoter) | This work |
pRKC0628 | pCN51_Teg41_3′(vector overexpressing the 3′ end of Teg41 from an inducible promoter) | This work |
pJB38 | Temperature-sensitive allelic exchange plasmid (Cmr) | 46 |
pJB1037 | pJB38 containing fragment upstream of Teg41 | This work |
pJB1039 | pJB1037 containing fragment downstream of Teg41 | This work |