TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic | Reference or source |
|---|---|---|
| S. aureus strains | ||
| RN4220 | Restriction-deficient transformation recipient | 36 |
| TCH1516 | Community-associated USA300 MRSA isolate | 37 |
| UAMS-1 | Osteomyelitis clinical isolate | 38 |
| SH1000 | rsbU repaired laboratory strain | 39 |
| Newman | Laboratory strain | 40 |
| AH1263 | USA300 LAC isolate cured of plasmid LAC-p03 | 41 |
| JE2 | USA300 LAC isolate cured of plasmids LAC-p01 and LAC-p03 | 42 |
| RKC0602 | UAMS-1 pMK4 | This work |
| RKC0603 | UAMS-1 pMK4_Teg41 | This work |
| RKC0600 | SH1000 pMK4 | This work |
| RKC0601 | SH1000 pMK4_Teg41 | This work |
| RKC0535 | AH1263 pMK4_Teg41 | This work |
| RKC0604 | Newman pMK4 | This work |
| RKC0605 | Newman pMK4_Teg41 | This work |
| RKC0072 | TCH1516 pMK4 | This work |
| RKC0474 | TCH1516 pMK4_Teg41 | This work |
| RKC0494 | JE2 pMK4 | This work |
| RKC0470 | JE2 pMK4_Teg41 | This work |
| RKC0599 | AH1263 pMK4 | This work |
| RKC0538 | AH1263 pMK4_Teg41 | This work |
| RKC0630 | AH1263 pCN51 | This work |
| RKC0614 | AH1263 pCN51_Teg41 | This work |
| RKC0628 | AH1263 pCN51_Teg41_3′ | This work |
| RKC0552 | AH1263 pMK4_Teg41_5′ | This work |
| NE1354 | USA300 JE2 hla::Bursa, hla NARSA transposon mutant | 42 |
| RKC0183 | TCH1516 hla::Bursa, hla mutant | This work |
| RKC0472 | TCH1516 hla::Bursa pMK4_Teg41 | This work |
| RKC0521 | AH1263 hla::Bursa, hla mutant | This work |
| BB2373 | JE2 Δpsmα | B. Boles |
| RKC0504 | JE2 Δpsmα pMK4 | This work |
| RKC0442 | JE2 Δpsmα pMK4_Teg41 | This work |
| JLB162 | AH1263 Teg41 3′ deletion (Teg41Δ3′) | This work |
| RKC0535 | JLB162 pMK4_Teg41 | This work |
| RKC0553 | JLB162 pMK4_Teg41_5′ | This work |
| RKC0631 | JLB162 pCN51 | This work |
| RKC0615 | JLB162 pCN51_Teg41 | This work |
| RKC0629 | JLB162 pCN51_Teg41_3′ | This work |
| S. epidermidis strains | ||
| 1457 | Wild-type S. epidermidis | 43 |
| RKC0518 | 1457 pMK4 | This work |
| RKC0519 | 1457 pMK4_Teg41 | This work |
| E. coli strain | ||
| DH5α | Cloning strain | Invitrogen |
| Plasmids | ||
| pMK4 | Gram-positive shuttle vector (Cmr) | 44 |
| pRKC0486 | pMK4_Teg41 (vector overexpressing Teg41 from its native promoter) | This work |
| pRKC0554 | pMK4_Teg41_5′ (vector overexpressing the 5′end of Teg41 from its native promoter, i.e., Teg41 missing the 3′ end) |
This work |
| pCN51 | Cadmium-inducible expression vector | 45 |
| pRKC0473 | pCN51_Teg41 (vector overexpressing Teg41 from an inducible promoter) | This work |
| pRKC0628 | pCN51_Teg41_3′(vector overexpressing the 3′ end of Teg41 from an inducible promoter) | This work |
| pJB38 | Temperature-sensitive allelic exchange plasmid (Cmr) | 46 |
| pJB1037 | pJB38 containing fragment upstream of Teg41 | This work |
| pJB1039 | pJB1037 containing fragment downstream of Teg41 | This work |