FIG 1.

Visualization and quantification of A3B expression in PyV-infected cells. (A and B) Immunofluorescent images and quantification of TAg, VP1, and A3B in BKPyV-infected HuK(i)G10 cells at 1 and 5 dpi (significance determined using Welch’s two-tailed t test; n = 2 biological replicates). (C) Time course of A3B mRNA levels in JCPyV (Mad1 strain) versus mock-infected HuK(i)G10 cells. (D) RT-qPCR quantification of A3B transcripts in mock-, BKPyV-, and JCPyV (Mad1)-infected HuK(i)G10 cells at 6 dpi (significance determined by Welch’s two-tailed t test; n = 3 technical replicates). (E) High-resolution immunofluorescent microscopy images of DAPI, A3B, EdU, and TAg in HuK(i)G10 cells infected with JCPyV (Mad1 strain). (F and G) Correlation coefficients and P values for EdU and A3B levels versus T antigen intensity in >100 cell images from a single experiment similar to that in panel E.