FIG 1.

Schematic showing the location of mutated TAC1, ERG11, MRR1, and UPC2 alleles on the respective chromosomes in the genetically engineered C. albicans strains used in this study. Genes (arrows) and chromosomes (gray lines) are not drawn to scale; only the relative positions of the genes, MTLa and MTLα loci (blue and green rectangles, respectively), and centromeres (black ovals) are indicated. Alleles carrying fluconazole resistance mutations (marked by stars) are represented by red arrows, and the corresponding wild-type alleles on the homologous chromosomes by white arrows. The polymorphic GLN3 and CAP1 alleles on the right arm of Chr5 and on the left arm of Chr3, respectively, which were used to analyze LOH events, are also shown and distinguished by color. Note that the parental strain SC5314 is almost completely homozygous for the right arm of Chr3; the putative location of the mutated MRR1 allele on Chr3A in strain SCMRR1R32B was deduced from its linkage to the CAP1-2 allele after an LOH event in homozygous derivatives; for strain SCMRR1R32A no such derivative was obtained, and the location of the mutated MRR1 on Chr3A or Chr3B in this strain has not been established.