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. 2019 Feb 5;10(1):e02816-18. doi: 10.1128/mBio.02816-18

FIG 6.

FIG 6

Wild-type macrophages contain larger CCVs but are less permissive for C. burnetii replication than TFE3/B knockout macrophages. (A) Representative fluorescence micrographs of control (Ctrl) or TFE3/B double-knockout (KO) RAW macrophages infected with C. burnetii. Cells were stained for DNA (blue), C. burnetii (red), and LAMP1 (green). (B) Quantitation of CCV size in control or TFE3/B double-knockout RAW macrophages 72 hpi (n = >100 cells). Data are representative of results from three independent experiments. (C) Replication of C. burnetii in control or TFE3/B double-knockout RAW macrophages at the indicated time points. The plot depicts means of fold increase ± standard deviations in C. burnetii genome equivalents (GE) relative to the mean at 4 hpi for three independent experiments. Asterisks indicate statistical significance (***, P < 0.001). Scale bar, 10 µm.