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. 2019 Feb 5;10(1):e02816-18. doi: 10.1128/mBio.02816-18

FIG 8.

FIG 8

Culture of THP-1 macrophages without glucose attenuates C. burnetii-mediated inhibition of mTORC1 and impairs bacterial replication. (A) Immunoblot of lysates from THP-1 macrophages left uninfected (UI) or infected with wild-type C. burnetii (WT) or the ΔdotA mutant for 72 h in Comp, AA−, or Gluc− medium and probed with antibodies against p4E-BP1 or actin. (B) Quantitation of p4E-BP1 signal in panel A. The plot depicts means ± standard deviations of p4E-BP1 signal normalized to the actin loading control relative to uninfected cells for three independent experiments. (C) Replication of C. burnetii in THP-1 macrophages incubated in Comp, AA−, or Gluc− medium. The plots depict means ± standard deviations of the fold change in bacterial genome equivalents (GE) relative to the mean at 4 hpi for three independent experiments. Asterisks indicate statistical significance (***, P < 0.001; ****, P < 0.0001).