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. 2019 Mar 15;13:50. doi: 10.3389/fnbeh.2019.00050

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Copyright © 2019 Murata, Kinoshita, Fukazawa, Kobayashi, Yamanaka, Hikida, Manabe and Yamaguchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell type-specific gene expression in dopamine receptor D1- and D2-expressing neurons in the anteromedial OT of the D1-Cre and D2-Cre mice using AAV vectors. (A,B) Confocal images of AAV-derived EYFP-expressing cells (green) and D1 (upper panels) or D2 (lower panels) mRNAs (red) from a D1-Cre (A) or D2-Cre (B) mouse. Color merged panel contains DAPI staining (blue). Scale bars: 20 μm. (C) Percentage of D1 or D2 mRNA-expressing cells among EYFP-expressing cells in D1-Cre and D2-Cre mice. Data show mean with SD. OT, olfactory tubercle; AAV, adeno-associated virus; EYFP, enhanced yellow fluorescent protein; D1, dopamine receptor D1; D2, dopamine receptor D2; DAPI, 4′,6-diamidino-2-phenylindole; SD, standard deviation.