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Frequency of methylation intensity in individual T-DNAs from a heterogeneous population over the 35S-eGFP+hp time course. The fully methylated PCR product amplified by the dBS F4.1 and dBS R7.1 Illumina fusion primers (Supplementary Table S1) contains 92 cytosine sites. Individual T-DNA reads are grouped based on their intensity by the number of methylated cytosines (mCs) into low (0, 1, and 2), medium (3–30 and 31–60) and high (61–92); n = 3 biological replicates, means ± SEM.
