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. 2019 Mar 21;9:4988. doi: 10.1038/s41598-019-41541-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of RNAi treatment of Se-AQP on hemocyte phagocytosis. (A) Effect of dsAQP on FITC-labeled E. coli. One microliter of heat-killed (HK) E. coli (~3.5 × 10⁴ cells/larva) were injected into larvae at 24 h after dsRNA treatment. A GFP gene was used as a control dsRNA (‘dsCON’). (B) Quantitative representation of phagocytosis between dsCON and dsAQP treated hemocytes. Each treatment was independently replicated three times. Asterisk mark (*) on bars indicates significant differences among means at Type I error = 0.05 (LSD test).