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. 2019 Mar 21;10:1298. doi: 10.1038/s41467-019-09199-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Xrn1 depletion leads to immediate defects on viral RNA2 translation. a Scheme of the experimental set-up to simultaneously deplete Xrn1-AID and express viral 2a-Rluc. b Expression of Xrn1-AID upon addition of auxin to the media. c UV absorbance profile at 260 nm of an extract from the Xrn1-AID strain before and after 35 min of auxin addition. d Relative RNA2-Rluc RNA accumulation, obtained by quantifying RNA level by RT-qPCR (arbitrary units (a.u)) and e Relative 2a-Rluc protein expression (R.U.) before and after galactose and auxin addition. Results represent averages obtained from n = 3 biological replicates. Error bars represent SEM. Source data are provided as a Source Data file